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The rapid design, assembly and validation of paper-based biomolecular sensors for the
portable detection of Zika virus is shown here. Using sequence information from online databases, primers for isothermal RNA amplification and toehold switch-based RNA sensors are designed in silico using algorithms. Sensors are embedded into paper discs and freeze-dried along with a cell-free transcription and translation system, to be deployed in the field as colorimetric diagnostics. In the diagnostic test, viral RNA is extracted, isothermally amplified, and used to rehydrate the freeze-dried sensors, resulting in a color change from yellow to purple in the presence of Zika RNA. Strain differentiation at single-base resolution is provided by a CRISPR-based module which connects to a secondary paper-based test that also provides colorimetric output.
| Date Taken: | 08.16.2016 |
| Date Posted: | 08.16.2016 12:07 |
| Photo ID: | 2795487 |
| VIRIN: | 160816-A-AB123-002 |
| Resolution: | 2237x2208 |
| Size: | 439.46 KB |
| Location: | FORT BELVOIR, VIRGINIA, US |
| Web Views: | 144 |
| Downloads: | 2 |
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Rock; Paper; Zika: Paper Wins as New Detection Tool
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