Tiny Tech With Big Impact

FORT BELVOIR, VA, UNITED STATES

09.12.2017

Courtesy Photo

Defense Threat Reduction Agency's Chemical and Biological Technologies Department

Increased local substrate concentrations in enzyme-DNA nanostructures as a result of engineered substrate-DNA interactions. Left: The DNA structures used here to investigate the effects of substrate-DNA binding interactions on enzyme catalysis. Colored strands are indicative of sequence. Right: Representation of enzyme-DNA nanostructures with a double-crossover DNA tile chemically conjugated to an HRP. The mechanism of increased local substrate concentration and enhanced catalysis is shown schematically: binding interactions between enzyme substrates and the DNA scaffold result in increased local concentrations of substrate, giving rise to a decrease in apparent Michaelis-Menten constant (KM) and a corresponding increase in enzyme efficiency (kcat/KM).

• Date Taken: 09.12.2017
• Date Posted: 09.12.2017 14:28
• Photo ID: 3759979
• VIRIN: 170912-A-AB123-001
• Resolution: 3175x1525
• Size: 527.82 KB
• Location: FORT BELVOIR, VA, US