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Increased local substrate concentrations in enzyme-DNA nanostructures as a result of engineered substrate-DNA interactions. Left: The DNA structures used here to investigate the effects of substrate-DNA binding interactions on enzyme catalysis. Colored strands are indicative of sequence. Right: Representation of enzyme-DNA nanostructures with a double-crossover DNA tile chemically conjugated to an HRP. The mechanism of increased local substrate concentration and enhanced catalysis is shown schematically: binding interactions between enzyme substrates and the DNA scaffold result in increased local concentrations of substrate, giving rise to a decrease in apparent Michaelis-Menten constant (KM) and a corresponding increase in enzyme efficiency (kcat/KM).
| Date Taken: | 09.12.2017 |
| Date Posted: | 09.12.2017 14:28 |
| Photo ID: | 3759979 |
| VIRIN: | 170912-A-AB123-001 |
| Resolution: | 3175x1525 |
| Size: | 527.82 KB |
| Location: | FORT BELVOIR, VIRGINIA, US |
| Web Views: | 50 |
| Downloads: | 2 |
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